Lepista sordida is a valuable edible mushroom rich in natural bioactive compounds.In the present study, a high-quality whole-genome of a domesticated strain of L.sordida was sequenced, revealing a 40.67 Mb genome in 13 contigs.
Phylogenetic analysis revealed that L.sordida is evolutionarily closely related to edible mushroom Lyophyllum decastes and Hypsizygus marmoreus.Heat stress has a significant effect on the yield and quality of mushrooms, but the molecular basis for this is poorly understood in L.sordida.
A label-free comparative proteomic analysis was performed under different heat Trail Cans stress conditions.The growth of L.sordida mycelia was inhibited, and nuclear apoptosis occurred under heat stress.Ca2+ and MAPK signaling pathways were found to be involved in heat stress signal transduction.
It is hypothesized that the expression of various heat shock proteins plays a crucial role in the response to heat stress.In addition, the components of the ubiquitin-proteasome system and the thioredoxin system were upregulated, preventing the accumulation of misfolded proteins and possibly supporting the response to heat stress.In Female Contraceptives summary, these results provide a fundamental insight into the evolution and heat stress-responsive mechanisms in L.sordida and may facilitate the breeding of heat-tolerant strains for artificial cultivation.